Purpose: The current study was designed to investigate the expression pattern of chemokine in musculoskeletal tumors, and between primary osteosarcoma and recurred, and postchemotherapy one.
Materials and methods: Ten primary soft tissue and bone tumors, one primary, one recurred, one post-chemotherapy osteosarcoma, and one normal control patients were included in the current study. RT-PCR and RPA were used for the investigation of the expression of cytokines and chemokines. Fisher’s exact test in SPSS was used for the statistical analysis.
Results: IL-8 and TNF-αwere expressed in all tumor tissues, IFN-γwas in all except two cases, RANTES was in 5 soft tissue tumors and 4 bone tumors, GRO-αwas in one soft tissue tumor and 2 bone tumors, and MCP-1 and IP-10 were in two bone tumors and in all the other group. In recurred osteosarcoma all the cytokines and chemokines were expressed, and the degree of the expression was stronger than the primary, except IFN-γ. After chemotherapy, RANTES, IFN-βand TGFβ1 among the TGFβisoforms were expressed.
Conclusion: There were differences in the expression of cytokines and chemokines in some different bone and soft tissue tumors, even though it was impossible to support this statistically due to small numbers of cases. The expression pattern of IFN-γand TGF-βisoform in osteosarcoma could be used for the study of tumor recurrence and the changes after chemotherapy.
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